Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX49
  • Specificity
    ab33607 recognises DDX49.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    A region within synthetic peptide: LQCKASVKVV SKFSNSNKRK TRHTGEKHFK ECGKSFQKFS HLTQHKVIHA, corresponding to N terminal amino acids 145-194 of Human DDX49

  • Positive control
    • Fetal thymus lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 54 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

Target

Anti-DDX49 antibody images

  • Anti-DDX49 antibody (ab33607) at 1/2.5 dilution + Fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at a dilution of 1/50000 - 1/100000

    Predicted band size : 54 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References for Anti-DDX49 antibody (ab33607)

ab33607 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab33607.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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