Overview

  • Product name
    Anti-DDX5 antibody [2257C3a]
    See all DDX5 primary antibodies
  • Description
    Mouse monoclonal [2257C3a] to DDX5
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment of human DDX5.

  • Positive control
    • HeLa whole cell lysate, NIH3T3 whole cell lysate and F2408 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab53216 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    2257C3a
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function
    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Images

  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + F2408 whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    anti-Mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-DDX5 antibody [2257C3a] (ab53216) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

References

This product has been referenced in:
  • Pettersson OJ  et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). Read more (PubMed: 24792155) »
  • Yao H  et al. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA. Genes Dev 24:2543-55 (2010). Read more (PubMed: 20966046) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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