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Goat polyclonal to DDX5
Mouse, Rat, Human
Predicted to work with:
Dog, Non human primates
Synthetic peptide: PMIGYPMPTGYSQ, corresponding to C terminal amino acids 602-614 of Human DDX5.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).Can be blocked with
Human DDX5 peptide (ab23200).
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent dilution. See Abreview submitted by Reinhard Kodym.
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Nucleus > nucleolus.
Information by UniProt
ATP dependent RNA helicase DDX5 antibody
DDX 5 antibody
Anti-DDX5 antibody images
Immunocytochemistry/ Immunofluorescence - DDX5 antibody (ab10261)
HT1080 cells were fixed with paraformaldehyde permeabilized with Triton (1%) and stained for 1 hr in a 1/100 dilution of the DDX5 antibody and thereafter for 1 hr with a rhodamine conjugated anti-goat secondary. This image was kindly supplied as part of the review submitted by Reinhard Kodym.
Western blot - DDX5 antibody (ab10261)
Predicted band size : 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab10261)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue, staining DDX5 with ab10261 at 2.5 µg/ml. Staining was detected using Alkaline-Phosphatase .
References for Anti-DDX5 antibody (ab10261)
This product has been referenced in:
et al. The TLX-miR-219 cascade regulates neural stem cell proliferation in neurodevelopment and schizophrenia iPSC model. Nat Commun 7:10965 (2016).
Read more (PubMed: 26965827) »
et al. Specific residues of PB2 and PA influenza virus polymerase subunits confer the ability for RNA polymerase II degradation and virus pathogenicity in mice. J Virol 88:3455-63 (2014).
Read more (PubMed: 24403580) »
See all 12 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"