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Goat polyclonal to DDX5
Mouse, Rat, Human
Predicted to work with:
Dog, Non human primates
Synthetic peptide: PMIGYPMPTGYSQ, corresponding to C terminal amino acids 602-614 of Human DDX5.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).Can be blocked with
Human DDX5 peptide (ab23200).
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent dilution. See Abreview submitted by Reinhard Kodym.
RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Nucleus > nucleolus.
Information by UniProt
ATP dependent RNA helicase DDX5 antibody
DDX 5 antibody
Anti-DDX5 antibody images
Immunocytochemistry/ Immunofluorescence - DDX5 antibody (ab10261)
HT1080 cells were fixed with paraformaldehyde permeabilized with Triton (1%) and stained for 1 hr in a 1/100 dilution of the DDX5 antibody and thereafter for 1 hr with a rhodamine conjugated anti-goat secondary. This image was kindly supplied as part of the review submitted by Reinhard Kodym.
Western blot - DDX5 antibody (ab10261)
Predicted band size : 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody (ab10261)
Immunohistochemical analysis of paraffin-embedded Human prostate tissue, staining DDX5 with ab10261 at 2.5 µg/ml. Staining was detected using Alkaline-Phosphatase .
References for Anti-DDX5 antibody (ab10261)
This product has been referenced in:
et al. Specific residues of PB2 and PA influenza virus polymerase subunits confer the ability for RNA polymerase II degradation and virus pathogenicity in mice. J Virol 88:3455-63 (2014).
Read more (PubMed: 24403580) »
et al. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways. Nucleic Acids Res N/A:N/A (2013).
Read more (PubMed: 24275493) »
See all 10 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"