Overview

  • Product name
  • Description
    Mouse polyclonal to DDX5
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full-length Human DDX5 protein (AAH16027)

  • Positive control
    • DDX5 transfected 293T lysate, Hela nuclear extract, Raw 264.7 cell lysate and Hela cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications
      Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX5 antibody
      • DDX 5 antibody
      • Ddx5 antibody
      • DDX5_HUMAN antibody
      • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
      • DEAD box 5 antibody
      • DEAD box protein 5 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
      • G17P1 antibody
      • HELR antibody
      • HLR1 antibody
      • HUMP68 antibody
      • P68 antibody
      • p68 RNA helicase antibody
      • Probable ATP dependent RNA helicase DDX5 antibody
      • Probable ATP-dependent RNA helicase DDX5 antibody
      • RNA helicase p68 antibody
      see all

    Anti-DDX5 antibody images

    • Anti-DDX5 antibody (ab92917) at 1/500 dilution + Hela nuclear extract at 50 µg

      Predicted band size : 69 kDa
    • Anti-DDX5 antibody (ab92917) at 1/500 dilution + Raw 264.7 cell lysate at 50 µg

      Predicted band size : 69 kDa
    • All lanes : Anti-DDX5 antibody (ab92917) at 1/500 dilution

      Lane 1 : DDX5 transfected lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 69 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescence analysis of HeLa cells labelled with ab92917 at 10 µg/ml.

    References for Anti-DDX5 antibody (ab92917)

    ab92917 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab92917.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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