Recombinant
RabMAb

Anti-DDX5 antibody [EPR7239] (ab126730)

Overview

  • Product name
    Anti-DDX5 antibody [EPR7239]
    See all DDX5 primary antibodies
  • Description
    Rabbit monoclonal [EPR7239] to DDX5
  • Tested applications
    Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human DDX5 (UniProt P17844).

  • Positive control
    • HeLa, SH-SY5Y, A431, and HT29 cell lysates; Human bladder carcinoma and Human kidney tissues; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7239
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500.
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

Target

  • Function
    RNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Images

  • All lanes : Anti-DDX5 antibody [EPR7239] (ab126730) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 69 kDa
  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (human neuroblastoma) cells labelling DDX5 (green) with purified ab126730 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab126730, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab126730, at a dilution of 1/100, staining DDX5 in HeLa cells by Immunofluorescence.

References

ab126730 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab126730.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up