Overview

  • Product nameAnti-DDX5 (phospho Y593) antibody
    See all DDX5 primary antibodies
  • Description
    Rabbit polyclonal to DDX5 (phospho Y593)
  • Specificityab62255 detects endogenous levels of DDX5 only when phosphorylated at tyrosine 593.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human DDX5 around the phosphorylation site of tyrosine 593 (Q-A-YP-A-Y).

  • Positive control
    • HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab62255 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionRNA-dependent ATPase activity. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. May be involved in pre-mRNA splicing.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX5 antibody
    • DDX 5 antibody
    • Ddx5 antibody
    • DDX5_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box helicase 5 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 5 antibody
    • DEAD box 5 antibody
    • DEAD box protein 5 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD) antibody
    • G17P1 antibody
    • HELR antibody
    • HLR1 antibody
    • HUMP68 antibody
    • P68 antibody
    • p68 RNA helicase antibody
    • Probable ATP dependent RNA helicase DDX5 antibody
    • Probable ATP-dependent RNA helicase DDX5 antibody
    • RNA helicase p68 antibody
    see all

Anti-DDX5 (phospho Y593) antibody images

  • ab62255 at 1/500 - 1/1000 dilution staining DDX5 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References for Anti-DDX5 (phospho Y593) antibody (ab62255)

This product has been referenced in:
  • Arun G  et al. mrhl RNA, a long noncoding RNA, negatively regulates Wnt signaling through its protein partner Ddx5/p68 in mouse spermatogonial cells. Mol Cell Biol 32:3140-52 (2012). Read more (PubMed: 22665494) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 0.2 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Purified protein (purified protein)
Specification purified protein
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Ms. Christina George

Verified customer

Submitted Oct 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"