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Rabbit monoclonal [EPR5273] to DDX50
, ICCmore detailsUnsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human DDX50 aa 600-700. The exact sequence is proprietary.
- HeLa, 293T, K562 and Jurkat cell lysates; Human thyroid gland adenocarcinoma tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
||1/250 - 1/500.
Is unsuitable for Flow Cyt or IP.
Belongs to the DEAD box helicase family. DDX21/DDX50 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Nucleus > nucleolus.
Information by UniProt
- 4933429B04Rik antibody
- ATP-dependent RNA helicase DDX50 antibody
- Ddx50 antibody
Western blot - Anti-DDX50 antibody [EPR5273] (ab109515)
All lanes : Anti-DDX50 antibody [EPR5273] (ab109515) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX50 antibody [EPR5273] (ab109515)
Immunohistochemical analysis of DDX50 in paraffin embedded Human thyroid gland adenocarcinoma tissue, using ab109515 at a dilution of 1/100.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"