Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX52
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 49-549 of Human DDX52 (NP_008941.2)

  • Positive control
    • Whole cell lysate from HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab112027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Sequence similarities
      Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent RNA helicase ROK1 like antibody
      • ATP-dependent RNA helicase ROK1-like antibody
      • DDX52 antibody
      • DDX52_HUMAN antibody
      • DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 antibody
      • DEAD box protein 52 antibody
      • Human sequence similar to yeast 19 antibody
      • HUSSY19 antibody
      • Probable ATP dependent RNA helicase DDX52 antibody
      • Probable ATP-dependent RNA helicase DDX52 antibody
      • ROK1 antibody
      • ROK1, S. cerevisiae, homolog of antibody
      see all

    Images

    • Immunoprecipitation of DDX52. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Lane 1- DDXR52 was Immunoprecipitated by rabbit anti-DDX52 antibody (ab112027) at 6 µg/mg lysate. Lane 2- DDX52 was also immunoprecipitated by an rabbit anti-DDX52 antibody that recognizes a downstream epitope. Lane 3- Rabbit IgG control Western blot was performed with the same antibody as used in lane 2 at 0.4 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

    References

    ab112027 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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