Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX54
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 625 and 675 of human DDX54 (NP_076977.3)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410015A15Rik antibody
    • AI414901 antibody
    • ATP dependent RNA helicase DDX54 antibody
    • ATP-dependent RNA helicase DDX54 antibody
    • ATP-dependent RNA helicase DP97 antibody
    • DDX54 antibody
    • DDX54_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 antibody
    • DEAD box helicase 54 antibody
    • DEAD box protein 54 antibody
    • DEAD box RNA helicase 97 kDa antibody
    • DP97 antibody
    • MGC2835 antibody
    • RGD1562539 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX54 antibody (ab76947) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 180 kDa,230 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References

ab76947 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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