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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab76947 was affinity purified using an epitope specific to DDX54 immobilized on solid support.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
||Use at 2-5 µg/mg of lysate.
||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Nucleus > nucleolus.
Information by UniProt
- 2410015A15Rik antibody
- AI414901 antibody
- ATP dependent RNA helicase DDX54 antibody
Anti-DDX54 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX54 antibody (ab76947)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection: DAB.
Western blot - DDX54 antibody (ab76947)
All lanes : Anti-DDX54 antibody (ab76947) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 99 kDa
Observed band size : 99 kDa
Additional bands at : 180 kDa,230 kDa,72 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - DDX54 antibody (ab76947)
ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
References for Anti-DDX54 antibody (ab76947)
has not yet been referenced specifically in any publications.
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