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Rabbit polyclonal to DDX54
HumanPredicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 625 and 675 of human DDX54 (NP_076977.3)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab76947 was affinity purified using an epitope specific to DDX54 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
||Use at 2-5 µg/mg of lysate.
||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Has RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Nucleus > nucleolus.
Information by UniProt
- 2410015A15Rik antibody
- AI414901 antibody
- ATP dependent RNA helicase DDX54 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX54 antibody (ab76947)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-DDX54 antibody (ab76947)
All lanes : Anti-DDX54 antibody (ab76947) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 180 kDa, 230 kDa, 72 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-DDX54 antibody (ab76947)
ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
has not yet been referenced specifically in any publications.
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