Overview

  • Product nameAnti-DDX54 antibody
    See all DDX54 primary antibodies
  • Description
    Rabbit polyclonal to DDX54
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 625 and 675 of human DDX54 (NP_076977.3)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionHas RNA-dependent ATPase activity. Represses the transcriptional activity of nuclear receptors.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX54/DBP10 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410015A15Rik antibody
    • AI414901 antibody
    • ATP dependent RNA helicase DDX54 antibody
    • ATP-dependent RNA helicase DDX54 antibody
    • ATP-dependent RNA helicase DP97 antibody
    • DDX54 antibody
    • DDX54_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 antibody
    • DEAD box protein 54 antibody
    • DEAD box RNA helicase 97 kDa antibody
    • DP97 antibody
    • MGC2835 antibody
    • RGD1562539 antibody
    see all

Anti-DDX54 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX54 with ab76947 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX54 antibody (ab76947) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 180 kDa,230 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab76947 at 1µg/ml staining DDX54 in HeLa whole cell lysate immunoprecipitated using ab76947 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References for Anti-DDX54 antibody (ab76947)

ab76947 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab76947.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"