Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX55
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 175 and 225 of Human DDX55 (NP_065987.1)

  • Positive control
    • Whole cell lysate from HeLa or 293T cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Probable ATP-binding RNA helicase.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX55 antibody
    • ATP-dependent RNA helicase DDX55 antibody
    • DDX 55 antibody
    • ddx55 antibody
    • DDX55_HUMAN antibody
    • DEAD box protein 55 antibody
    • FLJ16577 antibody
    • KIAA1595 antibody
    • MGC33209 antibody
    see all

Images

  • All lanes : Anti-DDX55 antibody (ab109591) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 30 seconds
  • Detection of DDX55 by Western Blot of Immunprecipitate.
    ab109591 at 0.4µg/ml staining DDX55 in HeLa whole cell lysate immunoprecipitated using ab109591 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab109591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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