Overview

  • Product nameAnti-DDX56 antibody
    See all DDX56 primary antibodies
  • Description
    Rabbit polyclonal to DDX56
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 497-547 of Human DDX56 (Q9NY93).

  • Positive control
    • HeLa and 293T cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMay play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
  • Tissue specificityDetected in heart, brain, liver, pancreas, placenta and lung.
  • Sequence similaritiesBelongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 61 kd nucleolar helicase antibody
    • ATP-dependent 61 kDa nucleolar RNA helicase antibody
    • DDX21 antibody
    • DDX26 antibody
    • Ddx56 antibody
    • DDX56_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 56 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
    • DEAD box protein 21 antibody
    • DEAD box protein 56 antibody
    • DEAD box RNA helicase antibody
    • DEAD/H box 56 antibody
    • NOH61 antibody
    • Nucleolar helicase of 61 kDa antibody
    • Probable ATP-dependent RNA helicase DDX56 antibody
    • Putative nucleolar RNA helicase antibody
    see all

Anti-DDX56 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115158 at 1/1000 (1µg/ml). Detection: DAB.
  • Immunoprecipitation of DDX56 (3 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1-Immunoprecipitation with an anti-DDX56 antibody that recognizes an upstream epitope; Lane 2- Immunoprecipitation with anti-DDX56 antibody (ab115158); Lane 3- Immunoprecipitation with IgG control. Anti-DDX56 antibody (ab115158) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
  • All lanes : Anti-DDX56 antibody (ab115158) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg


    Predicted band size : 62 kDa


    Exposure time : 3 minutes

References for Anti-DDX56 antibody (ab115158)

ab115158 has not yet been referenced specifically in any publications.

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