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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
Use at 2-5 µg/mg of lysate.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Tissue specificity Detected in heart, brain, liver, pancreas, placenta and lung.
Sequence similarities Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization Nucleus > nucleolus.
Information by UniProt
61 kd nucleolar helicase antibody
ATP-dependent 61 kDa nucleolar RNA helicase antibody
Anti-DDX56 antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX56 antibody (ab115178)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115178 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-DDX56 antibody (ab115178)
All lanes : Anti-DDX56 antibody (ab115178) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 62 kDa Exposure time : 30 seconds
Immunoprecipitation - Anti-DDX56 antibody (ab115178)
Detection of DDX56 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.
References for Anti-DDX56 antibody (ab115178)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"