Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX56
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residue 300 and 350 of DDX56 according to NP_061955.1 (GeneID 54606).

  • Positive control
    • HeLa whole cell lysate, 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab115178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
  • Tissue specificity
    Detected in heart, brain, liver, pancreas, placenta and lung.
  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 61 kd nucleolar helicase antibody
    • ATP-dependent 61 kDa nucleolar RNA helicase antibody
    • DDX21 antibody
    • DDX26 antibody
    • Ddx56 antibody
    • DDX56_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box helicase 56 antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 antibody
    • DEAD box protein 21 antibody
    • DEAD box protein 56 antibody
    • DEAD box RNA helicase antibody
    • DEAD/H box 56 antibody
    • NOH61 antibody
    • Nucleolar helicase of 61 kDa antibody
    • Probable ATP-dependent RNA helicase DDX56 antibody
    • Putative nucleolar RNA helicase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling DDX56 with ab115178 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DDX56 antibody (ab115178) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 62 kDa


    Exposure time : 30 seconds
  • Detection of DDX56 by Immunoprecipitation analysis in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) using ab115178 for IP at 3 ug/mg lysate. Subsequence WB detection was performed using 1 ug/ml ab115178.

References

ab115178 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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