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Rabbit polyclonal to DDX56
HumanPredicted to work with:
Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 251-403 of Human DDX21 (AAH01235).
- A549, HeLa, HepG2 and HCT116 cell lines; MCF7 xenograft
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/100 - 1/500.
||1/500 - 1/3000. Predicted molecular weight: 62 kDa.
||1/100 - 1/200.
May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.
Detected in heart, brain, liver, pancreas, placenta and lung.
Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus > nucleolus.
Information by UniProt
- 61 kd nucleolar helicase antibody
- ATP-dependent 61 kDa nucleolar RNA helicase antibody
- DDX21 antibody
Western blot - DDX21 antibody (ab97648)
Anti-DDX56 antibody (ab97648) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - DDX21 antibody (ab97648)
ab97648, at a 1/200 dilution, staining DDX21 in paraformaldehyde fixed HeLa cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DDX21 antibody (ab97648)
ab97648, at a 1/500 dilution, staining DDX21 in formalin fixed, paraffin embedded MCF7 xenograft by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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