Overview

  • Product name
  • Description
    Goat polyclonal to DDX58
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human DDX58.

  • Positive control
    • Human brain cortex, placenta and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab111037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
  • Tissue specificity
    Present in vascular smooth cells (at protein level).
  • Sequence similarities
    Belongs to the helicase family.
    Contains 2 CARD domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Domain
    The repressor domain controls homomultimerization and interaction with MAVS.
    The helicase domain is responsible for dsRNA recognition.
    The 2 CARD domains are responsible for interaction with and signaling through MAVS.
    The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
  • Post-translational
    modifications
    Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
    Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
  • Cellular localization
    Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ddx58 antibody
    • DDX58_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
    • DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
    • DEAD box protein 58 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
    • DKFZp434J1111 antibody
    • DKFZp686N19181 antibody
    • FLJ13599 antibody
    • Probable ATP dependent RNA helicase DDX58 antibody
    • Probable ATP-dependent RNA helicase DDX58 antibody
    • Retinoic acid inducible gene 1 protein antibody
    • Retinoic acid-inducible gene 1 protein antibody
    • Retinoic acid-inducible gene I protein antibody
    • RIG I antibody
    • Rig-1 antibody
    • RIG-I antibody
    • RIG1 antibody
    • rigi antibody
    • RLR 1 antibody
    • RNA helicase antibody
    • RNA helicase RIG I antibody
    • SGMRT2 antibody
    see all

Images

  • ab111037, at 3.75µg/ml, staining DDX58 in formalin-fixed, paraffin-embedded Human brain cortex tissue by Immunohistochemistry.
  • ab111037, at 3.75µg/ml, staining DDX58 in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry.
  • ab111037, at 3.75µg/ml, staining DDX58 in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.

References

ab111037 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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