Overview

  • Product name
    Anti-DDX59 antibody
  • Description
    Rabbit polyclonal to DDX59
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 569 and 619 of Human DDX59 (NP_001026895.2)

  • Positive control
    • HeLa or 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab109592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 69 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Sequence similarities
    Belongs to the DEAD box helicase family. DDX59 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HIT-type zinc finger.
  • Domain
    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
  • Information by UniProt
  • Database links
  • Alternative names
    • DDX 59 antibody
    • DDX59 antibody
    • DDX59_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 59 antibody
    • DEAD box protein 59 antibody
    • DKFZP564B1023 antibody
    • OTTHUMP00000033840 antibody
    • OTTHUMP00000033841 antibody
    • Probable ATP-dependent RNA helicase DDX59 antibody
    • RP11-92G12.2 antibody
    • Zinc finger HIT domain containing protein 5 antibody
    • Zinc finger HIT domain-containing protein 5 antibody
    • ZNHIT5 antibody
    see all

Images

  • All lanes : Anti-DDX59 antibody (ab109592) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 69 kDa


    Exposure time : 3 minutes
  • Detection of DDX59 by Western Blot of Immunprecipitate.
    ab109592 at 1µg/ml staining DDX59 in HeLa whole cell lysate immunoprecipitated using ab109592 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab109592 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109592.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up