Overview

  • Product name
  • Description
    Rabbit polyclonal to DDX6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-236 of Human DDX6 (P26196).

  • Positive control
    • A431, H1299, HeLa, HepG2 whole cell lysates; Ca922 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89% PBS, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Relevance
    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
  • Cellular localization
    Cytoplasm; P-body. Note: Processing bodies (PB).
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX6 antibody
    • ATP dependent RNA helicase p54 antibody
    • DDX6 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
    • DEAD box 6 antibody
    • DEAD box protein 6 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
    • FLJ36338 antibody
    • HLR2 antibody
    • Oncogene RCK antibody
    • P54 antibody
    • Probable ATP-dependent RNA helicase DDX6 antibody
    • RCK antibody
    see all

Images

  • Anti-DDX6 antibody (ab151984) at 1/1000 dilution + Hela whole cell lysate
    at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Ca922 xenograft tissue labeling DDX6 with ab151984 at 1/100.

References

ab151984 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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