Overview

  • Product nameAnti-DDX6 antibody
    See all DDX6 primary antibodies
  • Description
    Goat polyclonal to DDX6
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Guinea pig, Dog
  • Immunogen

    Synthetic peptide corresponding to Human DDX6 aa 471-483 (C terminal).
    Sequence:

    C-AEYHSEPVEDEKP


    Database link: P26196

  • Positive control
    • Jurkat cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesPeptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
    • Cellular localizationCytoplasm; P-body. Note: Processing bodies (PB).
    • Database links
    • Alternative names
      • ATP dependent RNA helicase DDX6 antibody
      • ATP dependent RNA helicase p54 antibody
      • DDX6 antibody
      • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
      • DEAD box 6 antibody
      • DEAD box protein 6 antibody
      • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
      • FLJ36338 antibody
      • HLR2 antibody
      • Oncogene RCK antibody
      • P54 antibody
      • Probable ATP-dependent RNA helicase DDX6 antibody
      • RCK antibody
      see all

    Anti-DDX6 antibody images

    • All lanes : Anti-DDX6 antibody (ab45869) at 0.3 µg/ml

      Lane 1 : Human Burkitt's lymphoma cell line
      Lane 2 : Human T cell leukemia cells from peripheral blood
      Lane 3 : Human liver hepatocellular carcinoma

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 54 kDa
    • Anti-DDX6 antibody (ab45869) at 0.3 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

      Predicted band size : 54 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      Primary incubation was 1 hour. Detected by chemiluminescence.

    References for Anti-DDX6 antibody (ab45869)

    This product has been referenced in:
    • Shen X  et al. Identification of novel SHPS-1-associated proteins and their roles in regulation of insulin-like growth factor-dependent responses in vascular smooth muscle cells. Mol Cell Proteomics : (2009). WB ; Pig . Read more (PubMed: 19299420) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab45869.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"