Overview

  • Product nameAnti-DDX6 antibody
    See all DDX6 primary antibodies
  • Description
    Rabbit polyclonal to DDX6
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: KKTLKLPPKD LRIKTSDVTS TKGNEFEDYC LKRELLMGIF EMGWEKPSPI, corresponding to N terminal amino acids 73-122 of Human DDX6

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab49336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

Target

  • RelevanceDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis and cellular growth and division. In the process of mRNA degradation, DDX6 may play a role in mRNA decapping. It forms a complex with DCP1A, DCP2, EDC3 and EDC4/HEDLS.
  • Cellular localizationCytoplasm; P-body. Note: Processing bodies (PB).
  • Database links
  • Alternative names
    • ATP dependent RNA helicase DDX6 antibody
    • ATP dependent RNA helicase p54 antibody
    • DDX6 antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 6 antibody
    • DEAD box 6 antibody
    • DEAD box protein 6 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD) antibody
    • FLJ36338 antibody
    • HLR2 antibody
    • Oncogene RCK antibody
    • P54 antibody
    • Probable ATP-dependent RNA helicase DDX6 antibody
    • RCK antibody
    see all

Anti-DDX6 antibody images

  • Anti-DDX6 antibody (ab49336) at 1.25 µg/ml + HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-DDX6 antibody (ab49336)

ab49336 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab49336.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"