The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/150 - 1/500.
Coat with 1ug antigen per well. Use AP conjugated anti-sheep for detection.
Is unsuitable for IHC and WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May affect the rate of fibrils formation.
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Secreted > extracellular space > extracellular matrix.