The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration. PubMed: 20801503
May affect the rate of fibrils formation.
Involvement in disease
Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Secreted > extracellular space > extracellular matrix.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody (ab54728)Hallgren O et al., Respir Res. 2010; 11(1): 55. Fig 1., doi: 10.1186/1465-9921-11-55
ab54728 staining Decorin in Human lung tissue sections from chronic obstructive pulmonary disease (COPD) patients by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Panel G represents micrographs from the central airways (bronchi) and panel H shows the small airways and parenchyma. L denotes lumen of the bronchi, and A and V denote airway and vessels, respectively. Black arrowheads show staining in the lamina reticularis. Scale bars represent 100 μm.
Tissue was fixed with 4% paraformaldehyde and blocked with 2% BSA-TBS for 30 minutes. Samples were incubated with primary antibody and a Biotin-conjugated Horse anti-mouse polyclonal was used as the secondary antibody. Nuclei was stained blue with hematoxylin
Western blot - Decorin antibody (ab54728)
Western blot against tagged recombinant protein immunogen using ab54728 Decorin antibody at 1ug/ml. Predicted band size of immunogen is 65 kDa
Soria-Valles C et al. The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21. OncogeneN/A:N/A (2013).
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