The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration. PubMed: 20801503
FunctionMay affect the rate of fibrils formation.
Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. Contains 12 LRR (leucine-rich) repeats.
Post-translational modificationsThe attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Cellular localizationSecreted > extracellular space > extracellular matrix.
Decorin antibody (ab54728) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human adrenal gland.
Western blot - Decorin antibody (ab54728)
Western blot against tagged recombinant protein immunogen using ab54728 Decorin antibody at 1ug/ml. Predicted band size of immunogen is 65 kDa
References for Anti-Decorin antibody (ab54728)
This product has been referenced in:
Soria-Valles C et al. The anti-metastatic activity of collagenase-2 in breast cancer cells is mediated by a signaling pathway involving decorin and miR-21. OncogeneN/A:N/A (2013).
Read more (PubMed: 23851508) »