Anti-Decorin antibody (ab67449)

Overview

  • Product nameAnti-Decorin antibody
    See all Decorin primary antibodies
  • Description
    Rabbit polyclonal to Decorin
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, ChimpanzeeDoes not react with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Decorin.

    (Peptide available as ab71694.)

  • Positive control
    • This antibody gave a positive signal in Human adult skeletal muscle tissue lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab67449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionMay affect the rate of fibrils formation.
  • Involvement in diseaseDefects in DCN are the cause of congenital stromal corneal dystrophy (CSCD) [MIM:610048]. Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance.
  • Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bone proteoglycan II antibody
    • CSCD antibody
    • DCN antibody
    • DCN protein antibody
    • Decorin antibody
    • Decorin proteoglycan antibody
    • Dermatan sulphate proteoglycans II antibody
    • DKFZp686J19238 antibody
    • DSPG 2 antibody
    • DSPG2 antibody
    • PG 40 antibody
    • PG II antibody
    • PG S2 antibody
    • PG-S2 antibody
    • PG40 antibody
    • PGII antibody
    • PGS 2 antibody
    • PGS2 antibody
    • PGS2_HUMAN antibody
    • Proteoglycan core protein antibody
    • SLRR1B antibody
    • Small leucine rich protein 1B antibody
    see all

Anti-Decorin antibody images

  • Anti-Decorin antibody (ab67449) at 1 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa (possible isoform).
  • ab67449 staining Decorin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer pH 6. Samples were incubated with primary antibody (1/100 in 10% goat serum) for 16 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab67449 (1/250) staining Decorin in paraffin-embedded Human adrenal gland tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  The secondary antibody was goat anti rabbit conjugated to HRP.  For further experimental details please refer to abreview.

    See Abreview

References for Anti-Decorin antibody (ab67449)

ab67449 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Oligodendrocyte precursor cell line)
Permeabilization Yes - 0,5% Triton
Specification Oligodendrocyte precursor cell line
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, PH=6
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 26 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Specification skin
Fixative 10% Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ( PH=6)
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hongyan Zhao

Verified customer

Submitted Jan 10 2013

Thank you for contacting us.

The immunogen of antibody ab90425 shows 66.6% homology than ab67449 which shows 83% with mouse sequence. Based on this data I presume ab90425 would be suitable.

Please note we do not have tested these anti...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Skin)
Specification Skin
Fixative Formaldehyde
Permeabilization Yes - TBS 0.25% Tween for 10min at RT
Blocking step TBS 0.02% Triton X-100 5% donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Aug 15 2012

Thank you for your message. We appreciate your valuable feedback in the Abreviews.

I can confirm that you will need to place your order using the testing discount code forthe free of charge antibody before 20th August.

I hope this...

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Thank you foryour reply and for your patience.

I am very pleased to hear you would like to accept our offer and test ab67449 and ab5097in IHC-Fr and pig.

ab67449:
DISCOUNT CODE: ####
Expiration date: ####

ab50...

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Thank you very much for your telephone call and you interest in ab67449.

As discussed on the phone, to our knowledge, ab67449 has not been tested in IHC-Fr (frozen sections) or on pig samples.

Therefore, I can offer a discount off...

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DISCOUNT CODE: XXXXXXXX
Expiration date: 27 June 2012

I am very pleased to hear you would like to accept our offer and test ab67449 in Rat. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

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Thank you for contacting us.

Could you please provide the end user details e.g. their email address and institution details.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"