Anti-Decorin Binding Protein B antibody (ab106608)

Overview

  • Product name
    Anti-Decorin Binding Protein B antibody
  • Description
    Rabbit polyclonal to Decorin Binding Protein B
  • Specificity
    ab106608 is specific for Borrelia burgdorferi Decorin Binding Protein B. A BLAST analysis was used to suggest cross-reactivity with Decorin Binding Protein B from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Other
  • Immunogen

    MBP-fusion protein corresponding to Borrelia burgdorferi Decorin Binding Protein B.

  • Positive control
    • MBP-Decorin Binding Protein B fusion protein
  • General notes
    Store undiluted; dilute only prior to immediate use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab106608 was Protein A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab106608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 20 kDa.

Target

  • Relevance
    Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells.
  • Alternative names
    • dbpB antibody

Images

  • All lanes : Anti-Decorin Binding Protein B antibody (ab106608) at 1/1000 dilution

    Lane 1 : MBP-Decorin Binding Protein B fusion protein
    Lane 2 : MBP alone

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    HRP-conjugated Goat-Anti-Rabbit at 1/40000 dilution

    Predicted band size : 20 kDa

References

ab106608 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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