ab106608 is specific for Borrelia burgdorferi Decorin Binding Protein B. A BLAST analysis was used to suggest cross-reactivity with Decorin Binding Protein B from B. burgdorferi and B. garinii sources based on 100% homology with the immunizing sequence.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 20 kDa.
Decorin-binding protein B, or DbpB, binds to decorin, which may mediate the adherence of B.burgdorferi to collagen fibers in skin and other tissues. Spirochetal surface adhesions mediate attachment to decorin, a major component of the host extracellular matrix enabling bacteria to colonize in mammalian tissues. The spirochete migrates from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. Borrelia burgdorferi can colonize multiple tissues, and is capable of attachment to diverse cell types. The expression of decorin-binding protein (Dbp) A and/or DbpB, two B. burgdorferi surface proteins that bind GAGs, is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds 293 epithelial cells.
Western blot - Anti-Decorin Binding Protein B antibody (ab106608)
All lanes : Anti-Decorin Binding Protein B antibody (ab106608) at 1/1000 dilution
Lane 1 : MBP-Decorin Binding Protein B fusion protein Lane 2 : MBP alone
Lysates/proteins at 0.1 µg per lane.
Secondary All lanes : HRP-conjugated Goat-Anti-Rabbit at 1/40000 dilution
Predicted band size: 20 kDa
Gel concentration: 4-20%
Expected MWt of MBP-Decorin Binding Protein B fusion protein is 60.3 kDa.