Overview

  • Product name
    Anti-DECR1 antibody [8B1AD10]
    See all DECR1 primary antibodies
  • Description
    Mouse monoclonal [8B1AD10] to DECR1
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-P, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human liver mitochondria.

  • Positive control
    • MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HeLa cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
In-Cell ELISA Use a concentration of 4 µg/ml. (0.4 µg/well)
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Auxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
  • Tissue specificity
    Heart = liver = pancreas > kidney >> skeletal muscle = lung.
  • Sequence similarities
    Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2,4-dienoyl-CoA reductase subfamily.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1200012F07Rik antibody
    • 2 antibody
    • 2,4 dienoyl CoA reductase [NADPH] antibody
    • 2,4 dienoyl CoA reductase 1 antibody
    • 2,4 dienoyl CoA reductase mitochondrial antibody
    • 2,4-dienoyl-CoA reductase, mitochondrial antibody
    • 4 enoyl CoA reductase antibody
    • 4-dienoyl-CoA reductase [NADPH] antibody
    • 4-enoyl-CoA reductase [NADPH] antibody
    • DECR 1 antibody
    • DECR antibody
    • DECR_HUMAN antibody
    • DECR1 antibody
    • Nadph antibody
    • OTTMUSP00000004962 antibody
    • RP23-338J24.3 antibody
    • SDR18C1 antibody
    • Short chain dehydrogenase/reductase family 18C member 1 antibody
    see all

Images

  • DECR1 immunocaptured from human liver mitochondria using ab110287.
  • HeLa cells were stained with 1 µg/mL ab110287 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110287 staining DECR1 in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry. DECR1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.
  • MRC5 fibroblasts labeled with 1 µg/ml ab110287 and detected with goat anti-mouse secondary conjugated to FITC.

References

ab110287 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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