The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. This antibody will only immunoprecipitate DECR1 from isolated mitochondria.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
Use a concentration of 4 µg/ml. (0.4 µg/well)
Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionAuxiliary enzyme of beta-oxidation. It participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions. Catalyzes the NADP-dependent reduction of 2,4-dienoyl-CoA to yield trans-3-enoyl-CoA.
ab110287 staining DECR1 in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry. DECR1 immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.