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Tissue, cells or virus corresponding to Dectin-1.
ab53427 inhibits the binding of zymosan to macrophages via the beta glucan receptor. Fusion Partners: Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Our Abpromise guarantee covers the use of ab53427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.
The epitope recognised by 2A11 is sensitive to H2O2 treatment, therefore quenching with 0.3% H2O2 should be performed after incubation with the primary antibody.
|Flow Cyt||1/10 - 1/50.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.