Anti-Dectin-1 antibody [2A11] (FITC) (ab21646)

Overview

  • Product nameAnti-Dectin-1 antibody [2A11] (FITC)
    See all Dectin-1 primary antibodies
  • Description
    Rat monoclonal [2A11] to Dectin-1 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Dectin-1.

  • General notes


    This antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody inhibits the binding of zymosan to macrophages via the beta Glucan Receptor.
  • ClonalityMonoclonal
  • Clone number2A11
  • MyelomaY3/Ag1.2.3
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl for 106 cells in 100µl.





ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionLectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • Tissue specificityHighly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • Involvement in diseaseDefects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to glucan binding.
  • Cellular localizationCytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

Anti-Dectin-1 antibody [2A11] (FITC) images

  • Flow cytometry staining of mouse peripheral blood granulocytes with ab21646.

References for Anti-Dectin-1 antibody [2A11] (FITC) (ab21646)

ab21646 has not yet been referenced specifically in any publications.

Product Wall


Information we have from the clone originators indicates the antibody recognises both isoforms.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (RAW and J774 murine macrophages)
Specification RAW and J774 murine macrophages
Preparation Cell harvesting/tissue preparation method: routine cell culture --> scraped, washed
Sample buffer: PBS + FBS (3%)
Fixation None
Permeabilization No
Gating Strategy Size gating of murine RAW cells
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Abcam user community

Verified customer

Submitted May 23 2008

Thank you for your enquiry. The antibody is specific for Dectin-1. This is the only antibody we carry for this particular target. The references listed on the datasheet are general references pertaining to the target of the antibody. They are n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"