Anti-Dectin-1 antibody [GE2] - Low endotoxin, Azide free (ab82888)

Overview

  • Product name
    Anti-Dectin-1 antibody [GE2] - Low endotoxin, Azide free
    See all Dectin-1 primary antibodies
  • Description
    Mouse monoclonal [GE2] to Dectin-1 - Low endotoxin, Azide free
  • Specificity
    Human beta Glucagon Receptor has two major (ßGR-A and ßGR-B) and several minor isoforms. ßGR-A and ßGR-B differ by the presence and absence, respectively, of a stalk region. ab82888 (clone GE2) recognizes both isoform A and B.
  • Tested applications
    Suitable for: Inhibition Assay, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Human Dectin-1.

  • Positive control
    • Human peripheral blood monocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Endotoxin level: <0.01EU/ug
  • Clonality
    Monoclonal
  • Clone number
    GE2
  • Myeloma
    Sp2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt 1/25. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
  • Involvement in disease
    Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to glucan binding.
  • Cellular localization
    Cytoplasm and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta GR antibody
    • Beta-glucan receptor antibody
    • BGR antibody
    • C type calcium dependent carbohydrate recognition domain lectin superfamily member 12 antibody
    • C type lectin domain family 7 member a antibody
    • C-type lectin domain family 7 member A antibody
    • C-type lectin superfamily member 12 antibody
    • CANDF4 antibody
    • CLC7A_HUMAN antibody
    • CLEC7A antibody
    • CLEC7A protein antibody
    • Clecsf12 antibody
    • DC-associated C-type lectin 1 antibody
    • Dectin 1 antibody
    • DECTIN 1 receptor antibody
    • Dectin-1 antibody
    • Dectin1 antibody
    • Dendritic cell associated C type lectin 1 antibody
    • Dendritic cell-associated C-type lectin 1 antibody
    • Lectin like receptor 1 antibody
    • Putative transmembrane protein dectin 1 antibody
    see all

Anti-Dectin-1 antibody [GE2] - Low endotoxin, Azide free images

  • Flow cytometry analysis of A549 cells, staining beta Glucan Receptor (green) with ab82888.

    Cells were infected with swollen conidia of A. fumigatus 13073 at an MOI of 10 or incubated with PBS (Control) for 30 min. Subsequently, the cells were stained with isotype (IC) antibody or the primary antibody and analyzed by flow cytometry. A PE-conjugated goat anti-mouse IgG was used as the secondary antibody.

References for Anti-Dectin-1 antibody [GE2] - Low endotoxin, Azide free (ab82888)

This product has been referenced in:
  • Han X  et al. ß-1,3-Glucan-Induced Host Phospholipase D Activation Is Involved in Aspergillus fumigatus Internalization into Type II Human Pneumocyte A549 Cells. PLoS One 6:e21468 (2011). Flow Cyt, Inhibition Assay ; Human . Read more (PubMed: 21760893) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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