Mouse monoclonal [GE2] to Dectin-1 - Low endotoxin, Azide free
Human beta Glucagon Receptor has two major (ßGR-A and ßGR-B) and several minor isoforms. ßGR-A and ßGR-B differ by the presence and absence, respectively, of a stalk region.
ab82888 (clone GE2) recognizes both isoform A and B.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/25. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.
Highly expressed in peripheral blood leukocytes and dendritic cells. Detected in spleen, bone marrow, lung, muscle, stomach and placenta.
Involvement in disease
Defects in CLEC7A may be a cause of familial candidiasis type 4 (CANDF4) [MIM:613108]. It is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.
Contains 1 C-type lectin domain.
Phosphorylated on tyrosine residues in response to glucan binding.
Flow Cytometry - Anti-beta Glucan Receptor antibody [GE2] - Low endotoxin, Azide free (ab82888)Image from Han X et al., PLoS One. 2011;6(7):e21468. Epub 2011 Jul 8. Fig 6.; doi:10.1371/journal.pone.0021468; July 8, 2011, PLoS ONE 6(7): e21468
Flow cytometry analysis of A549 cells, staining beta Glucan Receptor (green) with ab82888.
Cells were infected with swollen conidia of A. fumigatus 13073 at an MOI of 10 or incubated with PBS (Control) for 30 min. Subsequently, the cells were stained with isotype (IC) antibody or the primary antibody and analyzed by flow cytometry. A PE-conjugated goat anti-mouse IgG was used as the secondary antibody.
Bao Z et al. Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells. BMC Microbiol15:161 (2015).
Read more (PubMed: 26268695) »
Kyrmizi I et al. Corticosteroids block autophagy protein recruitment in Aspergillus fumigatus phagosomes via targeting dectin-1/Syk kinase signaling. J Immunol191:1287-99 (2013).
Read more (PubMed: 23817424) »