Overview

  • Product name
    Anti-DECTIN 2 antibody [D2.11E4]
    See all DECTIN 2 primary antibodies
  • Description
    Rat monoclonal [D2.11E4] to DECTIN 2
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Dectin 2 (extracellular domain) Fc Fusion protein.

  • General notes


    This clone (D2.11E4) does not stain brain microglia or meningeal macrophages.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified from tissue culture supernatant.
  • Primary antibody notes
    This clone (D2.11E4) does not stain brain microglia or meningeal macrophages.
  • Clonality
    Monoclonal
  • Clone number
    D2.11E4
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

Target

  • Function
    Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.
  • Tissue specificity
    Expressed in lung, spleen, lymph node, leukocytes, bone marrow, tonsils and dendritic cells. Strongly expressed in purified monocytes and weakly in B-cells. In peripheral blood cells, preferentially expressed in plasmacytoids rather than myeloids.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Domain
    A short stretch of the intracellular domain (AA 8-14) proximal to the transmembrane domain is required for association with Fc receptor gamma chain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 6 member A antibody
    • C type lectin superfamily member 10 antibody
    • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 10 antibody
    • C-type lectin domain family 6 member A antibody
    • C-type lectin superfamily member 10 antibody
    • CLC6A_HUMAN antibody
    • CLEC4N antibody
    • CLEC4N, mouse, homolog of antibody
    • Clec6a antibody
    • CLECSF10 antibody
    • DC associated C type lectin 2 antibody
    • DC-associated C-type lectin 2 antibody
    • Dectin-2 antibody
    • Dectin2 antibody
    • Dendritic cell associated lectin 2 antibody
    • Dendritic cell-associated C-type lectin 2 antibody
    see all

References for Anti-DECTIN 2 antibody [D2.11E4] (ab34724)

This product has been referenced in:
  • Barrett NA  et al. Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J Exp Med 208:593-604 (2011). Flow Cyt ; Mouse . Read more (PubMed: 21357742) »
  • Barrett NA  et al. Dectin-2 recognition of house dust mite triggers cysteinyl leukotriene generation by dendritic cells. J Immunol 182:1119-28 (2009). Flow Cyt ; Mouse . Read more (PubMed: 19124755) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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