Anti-DECTIN 2 antibody [IMG3D1] (ab107572)

Overview

  • Product nameAnti-DECTIN 2 antibody [IMG3D1]
    See all DECTIN 2 primary antibodies
  • Description
    Mouse monoclonal [IMG3D1] to DECTIN 2
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to amino acids 75-125 of Human DECTIN 2 (Q6EIG7).

  • Positive control
    • Human, mouse and rat heart lysate, human Ramos cell lysate, human PBMCs and human spleen tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberIMG3D1
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. Use 0.5 ug/5x105 cells



ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

Target

  • FunctionBinds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.
  • Tissue specificityExpressed in lung, spleen, lymph node, leukocytes, bone marrow, tonsils and dendritic cells. Strongly expressed in purified monocytes and weakly in B-cells. In peripheral blood cells, preferentially expressed in plasmacytoids rather than myeloids.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • DomainA short stretch of the intracellular domain (AA 8-14) proximal to the transmembrane domain is required for association with Fc receptor gamma chain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C type lectin domain family 6 member A antibody
    • C type lectin superfamily member 10 antibody
    • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 10 antibody
    • C-type lectin domain family 6 member A antibody
    • C-type lectin superfamily member 10 antibody
    • CLC6A_HUMAN antibody
    • CLEC4N antibody
    • CLEC4N, mouse, homolog of antibody
    • Clec6a antibody
    • CLECSF10 antibody
    • DC associated C type lectin 2 antibody
    • DC-associated C-type lectin 2 antibody
    • Dectin-2 antibody
    • Dectin2 antibody
    • Dendritic cell associated lectin 2 antibody
    • Dendritic cell-associated C-type lectin 2 antibody
    see all

Anti-DECTIN 2 antibody [IMG3D1] images

  • All lanes : Anti-DECTIN 2 antibody [IMG3D1] (ab107572) at 2 µg/ml

    Lane 1 : Human heart lysate
    Lane 2 : Human heart lysate with immunizing peptide
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat heart lysate
    Lane 5 : Human Ramos cell lysate

    Secondary
    goat anti-mouse Ig HRP

    Predicted band size : 24 kDa
  • Cell surface flow cytometric analysis of DECTIN 2 expression by 5x10^5 human PBMCs using 0.5 µg of ab107572 in red and 0.5 µg of isotype control in green.
  • Immunohistochemical analysis of DECTIN 2 in formalin-fixed, paraffin-embedded human spleen tissue using ab107572 at 5 µg/ml.

References for Anti-DECTIN 2 antibody [IMG3D1] (ab107572)

ab107572 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience. I am pleased to hear that enzymatic antigen retrieval seemed to help. After discussing this with a colleague I also have the following suggestions: - The blocking conditions are quite stringent therefore it is worth...

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Thank you for your patience. I think is makes sense to investigate the problems with IHC-P to see whether some protocol modifications will get the product working successfully, as we don’t know whether it is suitable for GMA embedded tissues. Would...

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Thank you for calling us today. I am sorry to hear you have been experiencing problems with this product. We have not received any other complaints about this product therefore there may be a problem with the vial received. I would be happy to offer ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"