Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab38747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa.
    This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:8,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
    • Tissue specificity
      Broadly expressed in the immune system and can be detected in T and B-cells.
    • Sequence similarities
      Contains 1 PH domain.
    • Domain
      The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • Post-translational
      modifications
      Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
    • Cellular localization
      Cytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
    • Information by UniProt
    • Database links
    • Alternative names
      • DEF-6 antibody
      • Def6 antibody
      • DEFI6_HUMAN antibody
      • Differentially expressed in FDCP 6 homolog antibody
      • Differentially expressed in FDCP 6 homologue mouse antibody
      • IBP antibody
      • IRF4-binding protein antibody
      • Swap70 like adapter of T cells antibody
      see all

    Images

    • Anti-DEF6 antibody (ab38747) at 1 µg/ml + Whole tissue lysate prepared from human thymus at 35 µg
      Developed using the ECL technique

      Predicted band size : 74 kDa

    References

    ab38747 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab38747.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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