The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 74 kDa.
This antibody gave a positive result in ELISA against the immunizing peptide. Antibody detection limit dilution 1:8,000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
Tissue specificityBroadly expressed in the immune system and can be detected in T and B-cells.
Sequence similaritiesContains 1 PH domain.
DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
Post-translational modificationsTyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
Cellular localizationCytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.