Overview

  • Product nameAnti-DEF6 antibody
    See all DEF6 primary antibodies
  • Description
    Mouse monoclonal to DEF6
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-337 of Human DEF6

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.
    IHC-P: Use at a concentration of 3 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPhosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP-70 signaling.
    • Tissue specificityBroadly expressed in the immune system and can be detected in T and B-cells.
    • Sequence similaritiesContains 1 PH domain.
    • DomainThe PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.
    • Post-translational
      modifications
      Tyrosine-phosphorylated by tyrosine-protein kinase LCK in response to T-cell activation.
    • Cellular localizationCytoplasm. Cell membrane. Nucleus. Recruited to the plasma membrane upon binding phosphatidylinositol 3,4,5-trisphosphate.
    • Information by UniProt
    • Database links
    • Alternative names
      • DEF-6 antibody
      • Def6 antibody
      • DEFI6_HUMAN antibody
      • Differentially expressed in FDCP 6 homolog antibody
      • Differentially expressed in FDCP 6 homologue mouse antibody
      • IBP antibody
      • IRF4-binding protein antibody
      • Swap70 like adapter of T cells antibody
      see all

    Anti-DEF6 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57228 DEF6 antibody at 1ug/ml. Predicted band size of immunogen is 63 kDa

    References for Anti-DEF6 antibody (ab57228)

    ab57228 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (vero)
    Loading amount 20000 cells
    Specification vero
    Treatment cells expressing recombitant DEF6
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Jun 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"