Anti-Deformed Epidermal Autoregulatory Factor 1 antibody (ab50719)

Overview

  • Product nameAnti-Deformed Epidermal Autoregulatory Factor 1 antibody
    See all Deformed Epidermal Autoregulatory Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Deformed Epidermal Autoregulatory Factor 1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab50719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

Target

  • FunctionTranscription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.
  • Tissue specificityExpressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.
  • Sequence similaritiesContains 1 MYND-type zinc finger.
    Contains 1 SAND domain.
  • Cellular localizationCytoplasm. Nucleus. When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced; Nucleus. Cytoplasm. Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% and Secreted. Secreted in some cell types.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deaf1 antibody
    • DEAF1 transcription factor antibody
    • DEAF1_HUMAN antibody
    • Deformed epidermal autoregulatory factor 1 homolog antibody
    • Nuclear DEA1 related transcriptional regulator antibody
    • Nuclear DEAF-1-related transcriptional regulator antibody
    • NUDR antibody
    • SPN antibody
    • Suppressin antibody
    • Zinc finger MYND domain containing protein 5 antibody
    • Zinc finger MYND domain-containing protein 5 antibody
    • ZMYND5 antibody
    see all

Anti-Deformed Epidermal Autoregulatory Factor 1 antibody images

  • Lane 1 : marker
    Lane 2 : Anti-Deformed Epidermal Autoregulatory Factor 1 antibody (ab50719) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 59 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

References for Anti-Deformed Epidermal Autoregulatory Factor 1 antibody (ab50719)

ab50719 has not yet been referenced specifically in any publications.

Product Wall

Application ChIP
Sample Human Cell lysate - whole cell (Liposarcoma Cell Line)
Specification Liposarcoma Cell Line
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 37%
Detection step Semiquantitative PCR
Positive control Anti-Acetyl Histone H3 Antibody
Negative control Rabbit IgG Antibody
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Submitted Feb 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"