Anti-Deformed Epidermal Autoregulatory Factor 1 antibody - C-terminal (ab75722)

Overview

  • Product nameAnti-Deformed Epidermal Autoregulatory Factor 1 antibody - C-terminal
    See all Deformed Epidermal Autoregulatory Factor 1 primary antibodies
  • Description
    Rabbit polyclonal to Deformed Epidermal Autoregulatory Factor 1 - C-terminal
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of mouse Deformed Epidermal Autoregulatory Factor 1 .

  • Positive control
    • 293 cell lysate transiently transfected with the Deformed Epidermal Autoregulatory Factor 1 gene.

Properties

Applications

Our Abpromise guarantee covers the use of ab75722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 59 kDa.
ELISA 1/1000.

Target

  • FunctionTranscription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm.
  • Tissue specificityExpressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls.
  • Sequence similaritiesContains 1 MYND-type zinc finger.
    Contains 1 SAND domain.
  • Cellular localizationCytoplasm. Nucleus. When expressed alone, localizes mainly in the cytoplasm but, when expressed with isoform 1, nuclear localization is enhanced; Nucleus. Cytoplasm. Cytoplasmic in non-mucinous colorectal carcinoma. When expressed alone, localized almost exclusively in the nucleus but, when expressed with isoform 4, nuclear expression decreases to 32% and cytoplasmic expression increases by 270% and Secreted. Secreted in some cell types.
  • Information by UniProt
  • Database links
  • Alternative names
    • Deaf1 antibody
    • DEAF1 transcription factor antibody
    • DEAF1_HUMAN antibody
    • Deformed epidermal autoregulatory factor 1 homolog antibody
    • Nuclear DEA1 related transcriptional regulator antibody
    • Nuclear DEAF-1-related transcriptional regulator antibody
    • NUDR antibody
    • SPN antibody
    • Suppressin antibody
    • Zinc finger MYND domain containing protein 5 antibody
    • Zinc finger MYND domain-containing protein 5 antibody
    • ZMYND5 antibody
    see all

Anti-Deformed Epidermal Autoregulatory Factor 1 antibody - C-terminal images

  • All lanes : Anti-Deformed Epidermal Autoregulatory Factor 1 antibody - C-terminal (ab75722) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the Deformed Epidermal Autoregulatory Factor 1 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 59 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Deformed Epidermal Autoregulatory Factor 1 antibody - C-terminal (ab75722)

ab75722 has not yet been referenced specifically in any publications.

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