The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
DEGP2 protease belongs to the family of serine-type proteases. Analysis of the Arabidopsis genome revealed 14 genes encoding members of Deg family. At least four Deg proteases (Deg1, DEGP2, Deg5 and Deg8) are targeted to the chloroplast. An in vitro study, recombinant DEGP2 was demonstrated to cleave the D1 protein after exposure to high light intensity. However, a recent in vivo study with mutants lacking DEGP2 demonstrated that DEGP2 is not essential for D1 degradation.