Recombinant
RabMAb

Anti-DEGS1 antibody [EPR9681] (ab167169)

Overview

  • Product name
    Anti-DEGS1 antibody [EPR9681]
    See all DEGS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9681] to DEGS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DEGS1 (C terminal). The exact sequence is proprietary.

  • Positive control
    • 293T, T47D and HepG2 cell lysates; Human breast carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).
    • Cellular localization
      Endoplasmic reticulum and Mitochondrial
    • Database links
    • Alternative names
      • Degenerative spermatocyte homolog 1 antibody
      • Degenerative spermatocyte homolog 1 lipid desaturase antibody
      • Degenerative spermatocyte homolog lipid desaturase antibody
      • DEGS 1 antibody
      • DEGS antibody
      • Delta 4 desaturase sphingolipid 1 antibody
      • Des 1 antibody
      • DES1 antibody
      • Dihydroceramide desaturase antibody
      • FADS7 antibody
      • Membrane fatty acid (lipid) desaturase antibody
      • Membrane lipid desaturase antibody
      • MGC5079 antibody
      • MIG15 antibody
      • Migration inducing gene 15 protein antibody
      • MLD antibody
      • Sphingolipid delta 4 desaturase antibody
      • Sphingolipid delta(4) desaturase DES1 antibody
      see all

    Images

    • All lanes : Anti-DEGS1 antibody [EPR9681] (ab167169) at 1/1000 dilution

      Lane 1 : 293T cell lysates
      Lane 2 : T47D cell lysates
      Lane 3 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 38 kDa

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling DEGS1 with ab167169 at 1/50 dilution.

    References

    ab167169 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Unfortunately, this antibody failed all of our initial in-house validation assays in mouse samples. We would not recommend it for use in mouse samples.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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