• Product nameAnti-DEK antibody
    See all DEK primary antibodies
  • Description
    Rabbit polyclonal to DEK
  • Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    A 14-22 amino acid region within synthetic peptide: APAAEGEGTP TQPASEKEPE MPGPREESEE EEDEDDEEEE EEEKEKSLIV EGKRE, corresponding to N terminal amino acids 5-59 of Human DEK

  • Positive control
    • HepG2 cell lysate, hepatocytes and spleen cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab22826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA 1/62500.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionInvolved in chromatin organization.
  • Tissue specificityUbiquitous. Expressed at relatively high levels.
  • Involvement in diseaseA chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
  • Sequence similaritiesContains 1 SAP domain.
  • Post-translational
    Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
  • Cellular localizationNucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6S231E antibody
    • Dek antibody
    • DEK gene antibody
    • DEK oncogene antibody
    • DEK oncogene DNA binding antibody
    • DEK_HUMAN antibody
    • Protein DEK antibody
    see all

Anti-DEK antibody images

  • Anti-DEK antibody (ab22826) at 0.4 µg/ml + HepG2 whole cell lysate (ab7900)

    Donkey Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7083)

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab22826 at 4.0 - 8.0 ug/ml, staining human DEK in paraffin embedded liver tissue. Arrows indicate positively labelled cells (hepatocytes).
  • ab22826 at 4.0 - 8.0 ug/ml, staining human DEK in paraffin embedded thymus tissue. Arrows indicate positively labelled cells (spleen cells).
  • ICC/IF image of ab22826 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22826, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-DEK antibody (ab22826)

This product has been referenced in:
  • Babaei-Jadidi R  et al. FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation. J Exp Med 208:295-312 (2011). IHC-P ; Mouse . Read more (PubMed: 21282377) »

See 1 Publication for this product

Product Wall

Merci pour ces précisions. En effet, l'anti-DEK ab83099 est également un bon alternatif. Le numéro de commande de remplacement gratuit (une unité de ab83099) est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'ex...

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Je parle effectivement français. Je suis désolé d'apprendre que les 2 anti-DEK ab22826 et ab76975 que vous avez testés ne fonctionnent pas comme indiqué sur les fiches techniques respectives. Votre protocole me semble bon, je crains que vous ayez r...

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Thank you for taking time to complete our questionnaire and for contacting us. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spe...

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Thank you for your enquiry and your interest in our products. This antibody has been tested in Western blot, ELISA and ICC/IF in the Lab. However, we have not tested it in IHC-P yet in house, this application (IHC-P) is listed based on a specif...

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