Overview

  • Product name
  • Description
    Rabbit polyclonal to DEK
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    9aa synthetic peptide from the region between aa 178-198 of Human DEK

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Stabilization buffer
    Preservative: 0.02% Sodium Azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/500. Predicted molecular weight: 43 kDa.

Target

  • Function
    Involved in chromatin organization.
  • Tissue specificity
    Ubiquitous. Expressed at relatively high levels.
  • Involvement in disease
    A chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
  • Sequence similarities
    Contains 1 SAP domain.
  • Post-translational
    modifications
    Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
  • Cellular localization
    Nucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6S231E antibody
    • Dek antibody
    • DEK gene antibody
    • DEK oncogene antibody
    • DEK oncogene DNA binding antibody
    • DEK_HUMAN antibody
    • Protein DEK antibody
    see all

References for Anti-DEK antibody (ab74975)

This product has been referenced in:
  • Sandén C  et al. The DEK oncoprotein binds to highly and ubiquitously expressed genes with a dual role in their transcriptional regulation. Mol Cancer 13:215 (2014). CHIPseq ; Human . Read more (PubMed: 25216995) »

See 1 Publication for this product

Product Wall

According to our records, ab74975 was proving difficult to use in Western blot and we were in contact in order to help resolve the issue.
Looking at our correspondence, it appears that we are awaiting more details in order to help us better unders...

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Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
We had so far o...

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Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (Leukemia cell line)
Specification
Leukemia cell line
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Other
Positive control
Nuclear lysate of untreated U937 cells.
Username

Abcam user community

Verified customer

Submitted Jan 21 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (testis)
Specification
testis
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100 0,5%
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jan 16 2012

Thank you very much for your interest in ab74975. To our knowledge, ab74975 has not been tested in ChIP. I can also offer a discount off a future purchase if you buy ab74975 now, test it in ChIP and submit feedback to us in the form of an Abreview. It ...

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Merci pour ces précisions. En effet, l'anti-DEK ab83099 est également un bon alternatif. Le numéro de commande de remplacement gratuit (une unité de ab83099) est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'ex...

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Je parle effectivement français. Je suis désolé d'apprendre que les 2 anti-DEK ab22826 et ab76975 que vous avez testés ne fonctionnent pas comme indiqué sur les fiches techniques respectives. Votre protocole me semble bon, je crains que vous ayez r...

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Thank you for taking time to complete our questionnaire and for contacting us. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spe...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (knees - sections prepared from the whole knee)
Specification
knees - sections prepared from the whole knee
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X-100
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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