Overview

  • Product nameAnti-DEK antibody
    See all DEK primary antibodies
  • Description
    Rabbit polyclonal to DEK
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within residues corresponding to a region within amino acids 324-373 (ELKETIKKLL ASANLEEVTM KQICKKVYEN YPTYDLTERK DFIKTTVKEL) of human DEK (NP_003463).

  • Positive control
    • MCF7 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab83099 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

Target

  • FunctionInvolved in chromatin organization.
  • Tissue specificityUbiquitous. Expressed at relatively high levels.
  • Involvement in diseaseA chromosomal aberration involving DEK is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia (PubMed:1549122). Translocation t(6;9)(p23;q34) with NUP214/CAN (PubMed:1549122). It results in the formation of a DEK-NUP214 fusion gene (PubMed:1549122).
  • Sequence similaritiesContains 1 SAP domain.
  • Post-translational
    modifications
    Phosphorylated by CK2. Phosphorylation fluctuates during the cell cycle with a moderate peak during G(1) phase, and weakens the binding of DEK to DNA.
  • Cellular localizationNucleus. Enriched in regions where chromatin is decondensed or sparse in the interphase nuclei.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6S231E antibody
    • Dek antibody
    • DEK gene antibody
    • DEK oncogene antibody
    • DEK oncogene DNA binding antibody
    • DEK_HUMAN antibody
    • Protein DEK antibody
    see all

Anti-DEK antibody images

  • Anti-DEK antibody (ab83099) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References for Anti-DEK antibody (ab83099)

ab83099 has not yet been referenced specifically in any publications.

Product Wall

Merci pour ces précisions. En effet, l'anti-DEK ab83099 est également un bon alternatif. Le numéro de commande de remplacement gratuit (une unité de ab83099) est : *******. Vous recevrez prochainement un mail de confirmation comprenant les détails d'ex...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"