Human Predicted to work with:
Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide from a region within residues: TGCQLLVYNY QEYPTYPDSA FQVTTGYQLP VYNYQPFPAY PRSPFQVTAG , corresponding to internal sequence amino acids 180-229 of Deleted in azoospermia 4 (NP_065153).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceDeleted in azoospermia 4 is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that plays an essential role in spermatogenesis. It may act by binding to the 3'-UTR of mRNAs and regulating their translation.