Anti-delta 1 Catenin antibody (ab71138)

Overview

  • Product nameAnti-delta 1 Catenin antibody
    See all delta 1 Catenin primary antibodies
  • Description
    Rabbit polyclonal to delta 1 Catenin
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human delta 1 Catenin.

    (Peptide available as ab72863.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates: HeLa Nuclear Whole Cell EBS E14 TG2A Day 0 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab71138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificityExpressed in vascular endothelium.
  • Sequence similaritiesBelongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications
    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Anti-delta 1 Catenin antibody images

  • All lanes : Anti-delta 1 Catenin antibody (ab71138) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 108 kDa
    Additional bands at : 50 kDa,56 kDa,68 kDa,73 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab71138 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71138, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 5µg/ml.

References for Anti-delta 1 Catenin antibody (ab71138)

ab71138 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Specification Placenta
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Submitted May 19 2010

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