Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.
1/100 - 1/250.
Use at an assay dependent concentration.
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family. Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
Post-translational modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin with unpurified ab92514 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
References for Anti-delta 1 Catenin antibody [EPR357(2)] (ab92514)
This product has been referenced in:
Uehara Y et al. Inhibition of ß-catenin and STAT3 with a curcumin analog suppresses gastric carcinogenesis in vivo. Gastric CancerN/A:N/A (2014).
Read more (PubMed: 25331984) »
Mann KM et al. Sleeping Beauty mutagenesis reveals cooperating mutations and pathways in pancreatic adenocarcinoma. Proc Natl Acad Sci U S A109:5934-41 (2012).
Read more (PubMed: 22421440) »