Anti-delta 1 Catenin (phospho S268) antibody [EPR2380] (ab79545)

Overview

  • Product nameAnti-delta 1 Catenin (phospho S268) antibody [EPR2380]
    See all delta 1 Catenin primary antibodies
  • Description
    Rabbit monoclonal [EPR2380] to delta 1 Catenin (phospho S268)
  • Specificityab79545 only detects delta 1 Catenin phosphorylated on serine 268.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding serine 268 of Human delta 1 Catenin.

  • Positive control
    • HeLa cell lysate treated with Calyculin A. A431 cells. Human breast carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab79545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificityExpressed in vascular endothelium.
    • Sequence similaritiesBelongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications
      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Anti-delta 1 Catenin (phospho S268) antibody [EPR2380] images

    • All lanes : Anti-delta 1 Catenin (phospho S268) antibody [EPR2380] (ab79545) at 1/5000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 108 kDa
      Observed band size : 90-120 kDa (why is the actual band size different from the predicted?)
    • ab79545 at 1/100 dilution staining delta 1 Catenin in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
    • Immunofluorescent staining of delta 1 Catenin in A431 cells using ab79545 at 1/100 dilution.

    References for Anti-delta 1 Catenin (phospho S268) antibody [EPR2380] (ab79545)

    ab79545 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab79545.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"