The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/5000. Detects a band of approximately 90-120 kDa (predicted molecular weight: 108 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
Tissue specificityExpressed in vascular endothelium.
Sequence similaritiesBelongs to the beta-catenin family. Contains 10 ARM repeats.
DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
Post-translational modificationsPhosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.