Anti-delta 1 Catenin (phospho Y228) antibody (ab61026)

Overview

  • Product name
    Anti-delta 1 Catenin (phospho Y228) antibody
    See all delta 1 Catenin primary antibodies
  • Description
    Rabbit polyclonal to delta 1 Catenin (phospho Y228)
  • Specificity
    Detects endogenous levels of delta 1 Catenin only when phosphorylated at tyrosine 228
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide from around the phosphorylation site of tyrosine 228 (DNYPGS) of human delta 1 Catenin

  • Positive control
    • HUVEC cells Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • Function
    Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
  • Tissue specificity
    Expressed in vascular endothelium.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 10 ARM repeats.
  • Domain
    A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
  • Post-translational
    modifications
    Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
  • Cellular localization
    Cytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cadherin associated Src substrate antibody
    • Cadherin-associated Src substrate antibody
    • CAS antibody
    • Catenin (cadherin associated protein) delta 1 antibody
    • Catenin delta 1 antibody
    • Catenin delta antibody
    • Catenin delta-1 antibody
    • CTND1_HUMAN antibody
    • CTNND 1 antibody
    • CTNND antibody
    • CTNND1 antibody
    • KIAA0384 antibody
    • p120 antibody
    • P120 CAS antibody
    • p120 catenin antibody
    • P120 CTN antibody
    • p120(cas) antibody
    • p120(ctn) antibody
    • P120CAS antibody
    • P120CTN antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab61026 at 1/50 dilution. Samples were treated -/+ immunising peptide.
  • All lanes : Anti-delta 1 Catenin (phospho Y228) antibody (ab61026) at 1/500 dilution

    Lane 1 : HUVEC extract
    Lane 2 : HUVEC extract with immunising peptide


    Predicted band size : 108 kDa
    Observed band size : 108 kDa
  • Immunofluorescence analysis of HUVECs labelled with ab61026 at 1/500 dilution. Left: untreated sample. Right: sample treated with immunising peptide.

References

ab61026 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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