Anti-delta 1 Catenin (phospho Y228) antibody [E205] (ab32403)

Overview

  • Product nameAnti-delta 1 Catenin (phospho Y228) antibody [E205]
    See all delta 1 Catenin primary antibodies
  • Description
    Rabbit monoclonal [E205] to delta 1 Catenin (phospho Y228)
  • SpecificityThis antibody only detects delta 1 Catenin phosphorylated on Tyrosine 228.
  • Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phospho peptide corresponding to residues surrounding Tyr 228 of human delta 1 Catenin.

  • Positive control
    • WB: HeLa cell lysate. IHC-P: Breast carcinoma.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 115 kDa (predicted molecular weight: 108 kDa).
IHC-P 1/50.
ICC 1/100.
IP 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionBinds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.
    • Tissue specificityExpressed in vascular endothelium.
    • Sequence similaritiesBelongs to the beta-catenin family.
      Contains 10 ARM repeats.
    • DomainA possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.
    • Post-translational
      modifications
      Phosphorylated by protein-tyrosine kinases. Dephosphorylated by PTPRJ.
    • Cellular localizationCytoplasm. Nucleus. Cell membrane. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cadherin associated Src substrate antibody
      • Cadherin-associated Src substrate antibody
      • CAS antibody
      • Catenin (cadherin associated protein) delta 1 antibody
      • Catenin delta 1 antibody
      • Catenin delta antibody
      • Catenin delta-1 antibody
      • CTND1_HUMAN antibody
      • CTNND 1 antibody
      • CTNND antibody
      • CTNND1 antibody
      • KIAA0384 antibody
      • p120 antibody
      • P120 CAS antibody
      • p120 catenin antibody
      • P120 CTN antibody
      • p120(cas) antibody
      • p120(ctn) antibody
      • P120CAS antibody
      • P120CTN antibody
      see all

    Anti-delta 1 Catenin (phospho Y228) antibody [E205] images

    • All lanes : Anti-delta 1 Catenin (phospho Y228) antibody [E205] (ab32403)

      Lane 1 : HeLa cell lysate, untreated.
      Lane 2 : HeLa cell lysate, treated with Pervanandate.
      Lane 3 : HeLa cell lysate, treated with Phosphatase.


      Predicted band size : 108 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    • Ab32403, at a 1/50 dilution, staining delta 1 Catenin in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.

    References for Anti-delta 1 Catenin (phospho Y228) antibody [E205] (ab32403)

    This product has been referenced in:
    • Ma LW  et al. Phosphorylated p120-catenin expression has predictive value for oral cancer progression. J Clin Pathol 65:315-9 (2012). Read more (PubMed: 22259181) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"