Overview

  • Product name
    Anti-delta 2 Catenin antibody
    See all delta 2 Catenin primary antibodies
  • Description
    Mouse monoclonal to delta 2 Catenin
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1081-1191 of Human delta 2 Catenin

Properties

Applications

Our Abpromise guarantee covers the use of ab54578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 133 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Functions as a transcriptional activator when bound to ZBTB33 (By similarity). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules.
  • Tissue specificity
    Predominantly expressed in brain.
  • Sequence similarities
    Belongs to the beta-catenin family.
    Contains 9 ARM repeats.
  • Post-translational
    modifications
    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cell junction > adherens junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARM-repeat protein antibody
    • Catenin (cadherin associated protein) delta 2 antibody
    • catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein) antibody
    • Catenin delta 2 antibody
    • Catenin delta-2 antibody
    • CTND2_HUMAN antibody
    • CTNND 2 antibody
    • Ctnnd2 antibody
    • Delta catenin antibody
    • Delta-catenin antibody
    • GT 24 antibody
    • GT24 antibody
    • Neural plakophilin related arm repeat protein antibody
    • Neural plakophilin related armadillo repeat protein antibody
    • Neural plakophilin-related ARM-repeat protein antibody
    • Neural plakophilin-related, Neurojungin antibody
    • Neurojungin antibody
    • NPRAP antibody
    • T cell delta catenin antibody
    see all

Anti-delta 2 Catenin antibody images

  • delta 2 Catenin antibody (ab54578) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 133 kDa
  • ICC/IF image of ab54578 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54578, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Overlay histogram showing SH-SY5Y cells stained with ab54578 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54578, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-delta 2 Catenin antibody (ab54578)

This product has been referenced in:
  • Kim H  et al. d-Catenin promotes E-cadherin processing and activates ß-catenin-mediated signaling: implications on human prostate cancer progression. Biochim Biophys Acta 1822:509-21 (2012). IHC-P ; Human . Read more (PubMed: 22261283) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (lung adenoma)
Specification
lung adenoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Oct 03 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung adenoma)
Specification
lung adenoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (endometrial adenocarcinoma)
Loading amount
25 µg
Specification
endometrial adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

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Verified customer

Submitted Sep 24 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (lung cancer primary tumor)
Loading amount
25 µg
Specification
lung cancer primary tumor
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Verified customer

Submitted Sep 24 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate)
Specification
prostate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
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Verified customer

Submitted Jul 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate)
Loading amount
50 µg
Specification
prostate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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