Overview

  • Product nameAnti-Delta Opioid Receptor antibody
    See all Delta Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Delta Opioid Receptor
  • SpecificityThis antibody detects endogenous levels of total Opioid Receptor delta protein.
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized non-phosphopeptide derived from human Delta Opioid Receptor around the phosphorylation site of serine 363 (T-P-SP-D-G).

  • Positive control
    • COS7 cell lysate or human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab63536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/40000.

Target

  • RelevanceFunction: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.
  • Cellular localizationMulti pass membrane protein
  • Database links
  • Alternative names
    • D-OR-1 antibody
    • Delta type opioid receptor antibody
    • Delta type opioid receptor DOR 1 antibody
    • Delta type opioid receptor DOR1 antibody
    • Delta-type opioid receptor antibody
    • DOR 1 antibody
    • DOR-1 antibody
    • DOR1 antibody
    • mDOR antibody
    • Nbor antibody
    • Opioid receptor delta 1 antibody
    • OPRD 1 antibody
    • OPRD antibody
    • OPRD_HUMAN antibody
    • Oprd1 antibody
    see all

Anti-Delta Opioid Receptor antibody images

  • All lanes : Anti-Delta Opioid Receptor antibody (ab63536) at 1/500 dilution

    Lane 1 : Extracts from COS7 cells (5-30ug), without immunising peptide.
    Lane 2 : Extracts from COS7 cells (5-30ug), with immunising peptide (5-10ug).


    Predicted band size : 40 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • Ab63536 (1/50-1/100) staining human Delta Opioid Receptor in human brain tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    The left hand panel is without immunising peptide, whereas the right hand panel is in the presence of immunising peptide.

References for Anti-Delta Opioid Receptor antibody (ab63536)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 400´l normal goat serum + 1600ml TBS + 0.1g bovine serum albumin (BSA) as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate 0.01M
Sample Rat Tissue sections (Colon)
Specification Colon
Permeabilization No
Fixative Paraformaldehyde
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Submitted Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"