Anti-Delta Opioid Receptor (phospho S363) antibody (ab62152)

Overview

  • Product name
    Anti-Delta Opioid Receptor (phospho S363) antibody
    See all Delta Opioid Receptor primary antibodies
  • Description
    Rabbit polyclonal to Delta Opioid Receptor (phospho S363)
  • Specificity
    Detects endogenous levels of Delta Opioid Receptor only when phosphorylated at serine 363
  • Tested applications
    Suitable for: IHC-P, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Delta Opioid Receptor around the phosphorylation site of serine 363 (T-P-SP-D-G).

  • Positive control
    • Brain

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/40000.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance
    Function: G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Tissue specificity: Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Similarity: Belongs to the G-protein coupled receptor 1 family. PTM: N-glycosylated. Ubiquitinated. A basal ubiquitination seems not to be related to degradation. Ubiquitination is increased upon formation of OPRM1:OPRD1 oligomers leading to proteasomal degradation; the ubiquitination is diminished by RTP4.
  • Cellular localization
    Multi pass membrane protein
  • Database links
  • Alternative names
    • D-OR-1 antibody
    • Delta type opioid receptor antibody
    • Delta type opioid receptor DOR 1 antibody
    • Delta type opioid receptor DOR1 antibody
    • Delta-type opioid receptor antibody
    • DOR 1 antibody
    • DOR-1 antibody
    • DOR1 antibody
    • mDOR antibody
    • Nbor antibody
    • Opioid receptor delta 1 antibody
    • OPRD 1 antibody
    • OPRD antibody
    • OPRD_HUMAN antibody
    • Oprd1 antibody
    see all

Images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using Delta Opioid Receptor (phospho S363) antibody (ab62152) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
  • ICC/IF image of ab62152 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62152, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Byun SW  et al. Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter. Cancer Lett 326:96-104 (2012). Read more (PubMed: 22867947) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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