Overview

  • Product nameAnti-delta Sarcoglycan antibody
    See all delta Sarcoglycan primary antibodies
  • Description
    Goat polyclonal to delta Sarcoglycan
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-RLPHGSYTPTGTR

    , corresponding to internal sequence amino acids 245-257 of Human delta Sarcoglycan(NP_000328.2; NP_758447.1; NP_001121681.1).

  • Positive control
    • Human and Rat heart lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab115019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).

Target

  • FunctionComponent of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • Tissue specificityMost strongly expressed in skeletal and cardiac muscle. Also detected in smooth muscle. Weak expression in brain and lung.
  • Involvement in diseaseDefects in SGCD are the cause of limb-girdle muscular dystrophy type 2F (LGMD2F) [MIM:601287]. LGMD2F is an autosomal recessive disorder.
    Defects in SGCD are the cause of cardiomyopathy dilated type 1L (CMD1L) [MIM:606685]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the sarcoglycan beta/delta/gamma/zeta family.
  • Post-translational
    modifications
    Glycosylated.
    Disulfide bonds are present.
  • Cellular localizationCell membrane > sarcolemma. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa dystrophin associated glycoprotein antibody
    • 35 kDa dystrophin-associated glycoprotein antibody
    • 35DAG antibody
    • CMD1L antibody
    • DAGD antibody
    • Delta-sarcoglycan antibody
    • Delta-SG antibody
    • Dystrophin associated glycoprotein delta sarcoglycan antibody
    • LGMD2F antibody
    • MGC22567 antibody
    • Placental delta sarcoglycan antibody
    • Sarcoglycan delta (35 kDa dystrophin associated glycoprotein) antibody
    • SG delta antibody
    • SGCD antibody
    • SGCD_HUMAN antibody
    • SGCDP antibody
    • SGD antibody
    see all

Anti-delta Sarcoglycan antibody images

  • Anti-delta Sarcoglycan antibody (ab115019) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour
  • ab115019 (3.8µg/ml) staining of paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Tissue treated: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-delta Sarcoglycan antibody (ab115019)

ab115019 has not yet been referenced specifically in any publications.

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