Overview

  • Product nameAnti-DeltaC antibody [zdc2]
  • Description
    Mouse monoclonal [zdc2] to DeltaC
  • SpecificityDelta proteins activate Notch through a binding reaction that depends on their extracellular domains. During somitogenesis, Notch signalling via DeltaC keeps the oscillations of neighbouring cells in synchrony. DeltaC is also expressed in subsets of cells in the nervous system and elsewhere.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Fr, Flow Cyt, IHC - Wholemount, ICC/IFmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Extracellular region of DeltaC protein (Zebrafish).

  • EpitopeDeltaC extracellular domain.
  • Positive control
    • HEK293 cells transfected with DeltaC or zebrafish embryo cryosections (formulin fixed).

Properties

Applications

Our Abpromise guarantee covers the use of ab73336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 73 kDa.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceDeltaC acts as a ligand for Notch receptors and is involved in somitogenesis. It can activate Notch receptors. It is required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized.
  • Cellular localizationMembrane; Single-pass type I membrane protein
  • Database links
  • Alternative names
    • Bea antibody
    • Beamter antibody
    • DelC antibody
    • Dlc antibody

References for Anti-DeltaC antibody [zdc2] (ab73336)

This product has been referenced in:
  • Quillien A  et al. Distinct Notch signaling outputs pattern the developing arterial system. Development 141:1544-52 (2014). Zebrafish . Read more (PubMed: 24598161) »
  • Giudicelli F  et al. Setting the tempo in development: an investigation of the zebrafish somite clock mechanism. PLoS Biol 5:e150 (2007). Read more (PubMed: 17535112) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Zebrafish Tissue sections (whole embryo)
Specification whole embryo
Username

Ms. Emerald Butko

Verified customer

Submitted Oct 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"