The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionActin-bundling protein. May function in mitogen-activated protein kinase pathway.
Tissue specificityHeart, brain, lung, skeletal muscle, and kidney.
Sequence similaritiesBelongs to the villin/gelsolin family. Contains 1 HP (headpiece) domain.
DomainContains at least two actin-binding sites, one in the headpiece domain and one in the amino-terminal portion. Consists of a large core fragment, the amino-terminal portion, and a small headpiece, the C-terminal portion. The headpiece can bind but cannot bundle actin filaments.
Post-translational modificationsActin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase. The N-terminus is blocked.