Anti-Dematin (phospho S403) antibody (ab61044)

Overview

  • Product nameAnti-Dematin (phospho S403) antibody
    See all Dematin primary antibodies
  • Description
    Rabbit polyclonal to Dematin (phospho S403)
  • SpecificityDetects Dematin only when phosphorylated at serine 403.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Dematin around the phosphorylation site of serine 403 (K-A-SP-L-F).

  • Positive control
    • Heart tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionActin-bundling protein. May function in mitogen-activated protein kinase pathway.
  • Tissue specificityHeart, brain, lung, skeletal muscle, and kidney.
  • Sequence similaritiesBelongs to the villin/gelsolin family.
    Contains 1 HP (headpiece) domain.
  • DomainContains at least two actin-binding sites, one in the headpiece domain and one in the amino-terminal portion.
    Consists of a large core fragment, the amino-terminal portion, and a small headpiece, the C-terminal portion. The headpiece can bind but cannot bundle actin filaments.
  • Post-translational
    modifications
    Actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase.
    The N-terminus is blocked.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DEMA antibody
    • DEMA_HUMAN antibody
    • Dematin antibody
    • DMT antibody
    • EPB49 antibody
    • erythrocyte membrane protein band 4.9 (dematin) antibody
    • Erythrocyte membrane protein band 4.9 antibody
    see all

Anti-Dematin (phospho S403) antibody images

  • All lanes : Anti-Dematin (phospho S403) antibody (ab61044)

    Lane 1 : HepG2 cells treated with insulin
    Lane 2 : phospho dematin


    Predicted band size : 46 kDa
  • 1/50-1/100 ab61044 staining phosphorilated Dematin on Human heart tissue, in the absence or presence of the immunizing phosphopeptide.

References for Anti-Dematin (phospho S403) antibody (ab61044)

ab61044 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"